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pcpgl basic reporter gene construct  (New England Biolabs)


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    New England Biolabs pcpgl basic reporter gene construct
    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of <t>the</t> <t>EBV</t> LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector <t>pCpGL-basic</t> and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.
    Pcpgl Basic Reporter Gene Construct, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgl+basic+reporter+gene+construct/pmc05309966-336-8-23?v=New+England+Biolabs
    Average 96 stars, based on 1060 article reviews
    pcpgl basic reporter gene construct - by Bioz Stars, 2026-08
    96/100 stars

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    1) Product Images from "Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency"

    Article Title: Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency

    Journal: Journal of Virology

    doi: 10.1128/JVI.01987-16

    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.
    Figure Legend Snippet: TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.

    Techniques Used: Activation Assay, Methylation, Modification, Clone Assay, Luciferase, Plasmid Preparation, In Vitro, Construct, Transfection, Activity Assay



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    pcpgl basic reporter gene construct  (New England Biolabs)
    96
    New England Biolabs pcpgl basic reporter gene construct
    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of <t>the</t> <t>EBV</t> LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector <t>pCpGL-basic</t> and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.
    Pcpgl Basic Reporter Gene Construct, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgl+basic+reporter+gene+construct/pmc05309966-336-8-23?v=New+England+Biolabs
    Average 96 stars, based on 1 article reviews
    pcpgl basic reporter gene construct - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

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    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.

    Journal: Journal of Virology

    Article Title: Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency

    doi: 10.1128/JVI.01987-16

    Figure Lengend Snippet: TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.

    Article Snippet: The EBV LMP1 ED-L1 promoter inserted into the pCpGL-basic reporter gene construct was methylated or mock treated in vitro using CpG methyltransferase M.SssI (New England BioLabs [NEB]) according the manufacturer's instructions.

    Techniques: Activation Assay, Methylation, Modification, Clone Assay, Luciferase, Plasmid Preparation, In Vitro, Construct, Transfection, Activity Assay